Human CHL1 ELISA Kit

Alternative name
Human close homolog of L1 ELISA Kit;Human CALL ELISA Kit;Human L1CAM2 ELISA Kit;Human cell adhesion molecule L1 like ELISA Kit;Human neural cell adhesion molecule L1-like protein ELISA Kit;Human L1 cell adhesion molecule 2 ELISA Kit;Human cell adhesion molecule with homology to L1CAM (close homolog of L1) ELISA Kit;Human cell adhesion molecule with homology to L1CAM (close homologue of L1) ELISA Kit;
Catalog
E007645
species
Human
GeneCHL1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cell Adhesion Molecule With Homology To L1CAM (CHL1). No significant cross-reactivity or interference between Cell Adhesion Molecule With Homology To L1CAM (CHL1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.058ng/mL.
Intended UseHuman CHL1 ELISA Kit allows for the in vitro quantitative determination of CHL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cell Adhesion Molecule With Homology To L1CAM (CHL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cell Adhesion Molecule With Homology To L1CAM (CHL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cell Adhesion Molecule With Homology To L1CAM (CHL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cell Adhesion Molecule With Homology To L1CAM (CHL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Processed neural cell adhesion molecule L1-like protein Protein information
Uniprot ID NCHL1_HUMAN
Uniprot AC O00533; B7ZL03; Q2M3G2; Q59FY0;
UniGene Hs.148909; Hs.731409;
GeneID 10752
KEGG hsa:10752;
Human Processed neural cell adhesion molecule L1-like protein Protein SEQUENCE
SEQUENCE 1208 AA; 135071 MW; F706F87B60A1685D CRC64;
MEPLLLGRGL IVYLMFLLLK FSKAIEIPSS VQQVPTIIKQ SKVQVAFPFD
EYFQIECEAK GNPEPTFSWT KDGNPFYFTD HRIIPSNNSG TFRIPNEGHI
SHFQGKYRCF ASNKLGIAMS EEIEFIVPSV PKFPKEKIDP LEVEEGDPIV
LPCNPPKGLP PLHIYWMNIE LEHIEQDERV YMSQKGDLYF ANVEEKDSRN
DYCCFAAFPR LRTIVQKMPM KLTVNSSNSI KQRKPKLLLP PTESGSESSI
TILKGEILLL ECFAEGLPTP QVDWNKIGGD LPKGRETKEN YGKTLKIENV
SYQDKGNYRC TASNFLGTAT HDFHVIVEEP PRWTKKPQSA VYSTGSNGIL
LCEAEGEPQP TIKWRVNGSP VDNHPFAGDV VFPREISFTN LQPNHTAVYQ
CEASNVHGTI LANANIDVVD VRPLIQTKDG ENYATVVGYS AFLHCEFFAS
PEAVVSWQKV EEVKPLEGRR YHIYENGTLQ INRTTEEDAG SYSCWVENAI
GKTAVTANLD IRNATKLRVS PKNPRIPKLH MLELHCESKC DSHLKHSLKL
SWSKDGEAFE INGTEDGRII IDGANLTISN VTLEDQGIYC CSAHTALDSA
ADITQVTVLD VPDPPENLHL SERQNRSVRL TWEAGADHNS NISEYIVEFE
GNKEEPGRWE ELTRVQGKKT TVILPLAPFV RYQFRVIAVN EVGRSQPSQP
SDHHETPPAA PDRNPQNIRV QASQPKEMII KWEPLKSMEQ NGPGLEYRVT
WKPQGAPVEW EEETVTNHTL RVMTPAVYAP YDVKVQAINQ LGSGPDPQSV
TLYSGEDYPD TAPVIHGVDV INSTLVKVTW STVPKDRVHG RLKGYQINWW
KTKSLLDGRT HPKEVNILRF SGQRNSGMVP SLDAFSEFHL TVLAYNSKGA
GPESEPYIFQ TPEGVPEQPT FLKVIKVDKD TATLSWGLPK KLNGNLTGYL
LQYQIINDTY EIGELNDINI TTPSKPSWHL SNLNATTKYK FYLRACTSQG
CGKPITEESS TLGEGSKGIG KISGVNLTQK THPIEVFEPG AEHIVRLMTK
NWGDNDSIFQ DVIETRGREY AGLYDDISTQ GWFIGLMCAI ALLTLLLLTV
CFVKRNRGGK YSVKEKEDLH PDPEIQSVKD ETFGEYSDSD EKPLKGSLRS
LNRDMQPTES ADSLVEYGEG DHGLFSEDGS FIGAYAGSKE KGSVESNGSS
TATFPLRA
UCSC uc003bot.4; human. [O00533-1];

Category

Human CHL1 ELISA Kit