Human CENPH ELISA Kit

Alternative name
Human CENP-H ELISA Kit;Human interphase centromere complex protein 35 ELISA Kit;Human ICEN35 ELISA Kit;Human centromere protein H ELISA Kit;Human kinetochore protein CENP-H ELISA Kit;
Catalog
E007735
species
Human
GeneCENPH
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CENPH. No significant cross-reactivity or interference between Human CENPH and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CENPH and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman CENPH ELISA Kit allows for the in vitro quantitative determination of CENPH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CENPH in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CENPH. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CENPH and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CENPH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CENPH. You can calculate the concentration of Human CENPH in the samples by comparing the OD of the samples to the standard curve.
Human Centromere protein H Protein information
Uniprot ID CENPH_HUMAN
Uniprot AC Q9H3R5; A8K3Y1;
UniGene Hs.631967;
GeneID 64946
KEGG hsa:64946;
Human Centromere protein H Protein SEQUENCE
SEQUENCE 247 AA; 28481 MW; E1BCD4022710168F CRC64;
MEEQPQMQDA DEPADSGGEG RAGGPPQVAG AQAACSEDRM TLLLRLRAQT
KQQLLEYKSM VDASEEKTPE QIMQEKQIEA KIEDLENEIE EVKVAFEIKK
LALDRMRLST ALKKNLEKIS RQSSVLMDNM KHLLELNKLI MKSQQESWDL
EEKLLDIRKK RLQLKQASES KLLEIQTEKN KQKIDLDSME NSERIKIIRQ
NLQMEIKITT VIQHVFQNLI LGSKVNWAED PALKEIVLQL EKNVDMM
UCSC uc003jvp.3; human.;

Category

Human CENPH ELISA Kit

Human CENP-H ELISA Kit;Human interphase centromere complex protein 35 ELISA Kit;Human ICEN35 ELISA Kit;Human centromere protein H ELISA Kit;Human kinetochore protein CENP-H ELISA Kit;