Bovine CS ELISA Kit

Alternative name
Chondroitin sulfate ELISA Kit,CS ELISA Kit
Catalog
E078778
species
Bovine
GeneCS
SpecificityNo cross-reaction with other factors
Sensitivity0.38 ng/mL
Intended UseBovine CS ELISA Kit allows for the in vitro quantitative determination of CS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole kit at 4℃
Product Description
specificalBovine CS ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to CS, During the reaction,CS in the sample or standard competes with a fixed amount of biotin-labeled CS for sites on a pre-coated Monoclonal antibody specific to CS. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Bovine Citrate synthase, mitochondrial Protein information
Uniprot ID CISY_BOVIN
Uniprot AC Q29RK1;
UniGene Bt.106917;
GeneID 280682
KEGG bta:280682;
Bovine Citrate synthase, mitochondrial Protein SEQUENCE
SEQUENCE 466 AA; 51773 MW; 73C2CB93589084B3 CRC64;
MALLTAAARL FGAKNASCLV LAARHASASS TNLKDILADL IPKEQTRVKA
FRQQHGKTVV GQITVDMMYG GMRGMKGLVY ETSVLDPDEG IRFRGYSIPE
CQKLLPKAKG GEEPLPEGLF WLLVTGQIPT EEQVSWLSQE WAKRAALPSH
VVTMLDNFPT NLHPMSQLSA AVTALNSEST FARAYSEGIN RTKYWELIYE
DSMDLIAKLP CVAAKIYRNL YREGSSIGAI DPKLDWSHNF TNMLGYTDAQ
FTELMRLYLT IHSDHEGGNV SAHTSHLVGS ALSDPYLSFA AAMNGLAGPL
HGLANQEVLV WLTQLQKEVG KDVSDEKLRD YIWNTLNSGR VVPGYGHAVL
RKTDPRYTCQ REFALKHLPQ DPMFKLVAQL YKIVPNILLE QGKAKNPWPN
VDAHSGVLLQ YYGMTEMNYY TVLFGVSRAL GVLAQLIWSR ALGFPLERPK
SMSTDGLMKF VDSKSG

Category

Bovine CS ELISA Kit

Chondroitin sulfate ELISA Kit,CS ELISA Kit