Rat CS ELISA Kit

Alternative name
Chondroitin sulfate ELISA Kit,CS ELISA Kit
Catalog
E078782
species
Rat
GeneCS
SpecificityNo cross-reaction with other factors
Sensitivity0.38 ng/mL
Intended UseRat CS ELISA Kit allows for the in vitro quantitative determination of CS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole kit at 4℃
Product Description
specificalRat CS ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to CS, During the reaction,CS in the sample or standard competes with a fixed amount of biotin-labeled CS for sites on a pre-coated Monoclonal antibody specific to CS. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Rat Citrate synthase, mitochondrial Protein information
Uniprot ID CISY_RAT
Uniprot AC Q8VHF5;
UniGene Rn.66581;
GeneID 170587
KEGG rno:170587;
Rat Citrate synthase, mitochondrial Protein SEQUENCE
SEQUENCE 466 AA; 51867 MW; AF8D38379CD38124 CRC64;
MALLTAAARL LGAKNSSCLV LAARHASASS TNLKDILSNL IPKEQARVKT
FRQQHGKTVV GQITVDMMYG GMRGMKGLVY ETSVLDPDEG IRFRGYSIPE
CQKLLPKAKG GEEPLPEGLF WLLVTGQMPT EEQVSWLSQE WAKRAALPSH
VVTMLDNFPT NLHPMSQLSA AITALNSESN FARAYAEGIN RTKYWELIYE
DCMDLIAKLP CVAAKIYRNL YREGSSIGAI DSKLDWSHNF TNMLCYTEPQ
FTELMRLYLT IHSDHDGGNV SAHTSHLVGS ALSDTYLSFA AAMNGLAGPL
HGLANQEVLV WLTQLQKEVG KDVSDEKLRD YIWNTLNSGR VVPGYGHAVL
RKTDPRYSCQ REFALKHLPK DPMFKLVAQL YKIVPNILLE QGKAKNPWPN
VDAHSGVLLQ YYGMTEMNYY TVLFGVSRAL GVLAQLIWSR ALGFPLERPK
SMSTDGLMKF VDSKSG
UCSC RGD:620330; rat.;

Category

Rat CS ELISA Kit

Chondroitin sulfate ELISA Kit,CS ELISA Kit