Human CFD ELISA Kit

Alternative name
Human ADIPSIN ELISA Kit;Human C3 convertase activator ELISA Kit;Human properdin factor D ELISA Kit;Human DF ELISA Kit;Human PFD ELISA Kit;Human ADN ELISA Kit;Human complement factor D ELISA Kit;Human D component of complement (adipsin) ELISA Kit;Human complement factor D preproprotein ELISA Kit;
Catalog
E007881
species
Human
GeneCFD
SpecificityNatural and recombinant human CFD
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman CFD ELISA Kit allows for the in vitro quantitative determination of CFD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human CFD ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CFD has been precoated onto 96-well plates. Standards(NSO, I26-A253) and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for CFD is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CFD amount of sample captured in plate. Background: Complement factor D (CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What's more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.
Human Complement factor D Protein information
Uniprot ID CFAD_HUMAN
Uniprot AC P00746; B4DV76; Q5U5S1; Q86VJ5; Q8N4E0; Q8WZB4;
UniGene Hs.155597;
GeneID 1675
KEGG hsa:1675;
Human Complement factor D Protein SEQUENCE
SEQUENCE 253 AA; 27033 MW; 78B06C209DEEA362 CRC64;
MHSWERLAVL VLLGAAACAA PPRGRILGGR EAEAHARPYM ASVQLNGAHL
CGGVLVAEQW VLSAAHCLED AADGKVQVLL GAHSLSQPEP SKRLYDVLRA
VPHPDSQPDT IDHDLLLLQL SEKATLGPAV RPLPWQRVDR DVAPGTLCDV
AGWGIVNHAG RRPDSLQHVL LPVLDRATCN RRTHHDGAIT ERLMCAESNR
RDSCKGDSGG PLVCGGVLEG VVTSGSRVCG NRKKPGIYTR VASYAAWIDS
VLA
UCSC uc002lqc.4; human.;

Category

Human CFD ELISA Kit

Human ADIPSIN ELISA Kit;Human C3 convertase activator ELISA Kit;Human properdin factor D ELISA Kit;Human DF ELISA Kit;Human PFD ELISA Kit;Human ADN ELISA Kit;Human complement factor D ELISA Kit;Human D component of complement (adipsin) ELISA Kit;Human complement factor D preproprotein ELISA Kit;