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Alternative name
Human eoCP ELISA Kit;Human eosinophil chemotactic cytokine ELISA Kit;Human SIS-delta ELISA Kit;Human Small-inducible cytokine A5 ELISA Kit;Human T cell-specific protein P228 ELISA Kit;Human TCP228 ELISA Kit;Human T-cell-specific protein RANTES ELISA Kit;Human D17S136E ELISA Kit;Human SCYA5 ELISA Kit;Human RANTES ELISA Kit;Human SISd ELISA Kit;Human C-C motif chemokine ligand 5 ELISA Kit;Human C-C motif chemokine 5 ELISA Kit;Human T-cell specific protein p288 ELISA Kit;Human beta-chemokine RANTES ELISA Kit;Human chemokine (C-C motif) ligand 5 ELISA Kit;Human regulated upon activation, normally T-expressed, and presumably secreted ELISA Kit;Human small inducible cytokine subfamily A (Cys-Cys), member 5 ELISA Kit;
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Catalog
E007972
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species
Human
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GeneCCL5
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human CCL5. No significant cross-reactivity or interference between human CCL5 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human CCL5 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
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Intended UseHuman CCL5 ELISA Kit allows for the in vitro quantitative determination of CCL5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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Human RANTES(4-68) Protein information
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Uniprot ID
CCL5_HUMAN
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Uniprot AC
P13501; O43646; Q0QVW8; Q4ZGJ1; Q9NYA2; Q9UBG2; Q9UC99;
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UniGene
Hs.514821;
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GeneID
6352
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KEGG
hsa:6352;
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Human RANTES(4-68) Protein SEQUENCE
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SEQUENCE 91 AA; 9990 MW; FB0BFAF9A87C620F CRC64;
MKVSAAALAV ILIATALCAP ASASPYSSDT TPCCFAYIAR PLPRAHIKEY
FYTSGKCSNP
AVVFVTRKNR QVCANPEKKW VREYINSLEM S
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UCSC
uc002hkf.5; human.;
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