Human CCKBR ELISA Kit

Alternative name
Human CCK-B receptor ELISA Kit;Human CCK-BR ELISA Kit;Human cholecystokinin-2 receptor ELISA Kit;Human CCK2-R ELISA Kit;Human CCKRB ELISA Kit;Human CCK-B ELISA Kit;Human CCK2R ELISA Kit;Human GASR ELISA Kit;Human cholecystokinin B receptor ELISA Kit;Human gastrin/cholecystokinin type B receptor ELISA Kit;Human CCK2 receptor ELISA Kit;Human gastrin receptor ELISA Kit;
Catalog
E008208
species
Human
GeneCCKBR
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CCKBR ELISA Kit allows for the in vitro quantitative determination of CCKBR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the Assay: CCKBR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCKBR antibody and an CCKBR-HRP conjugate. The assay sample and buffer are incubated together with CCKBR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCKBR concentration since CCKBR from samples and CCKBR-HRP conjugate compete for the anti-CCKBR antibody binding site. Since the number of sites is limited, as more sites are occupied by CCKBR from the sample, fewer sites are left to bind CCKBR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCKBR concentration in each sample is interpolated from this standard curve.
Human Gastrin/cholecystokinin type B receptor Protein information
Uniprot ID GASR_HUMAN
Uniprot AC P32239; A8K7P9; O75824; Q16144; Q92492; Q96LC6; Q9NYK7; Q9UBV1;
UniGene Hs.203;
GeneID 887
KEGG hsa:887;
Human Gastrin/cholecystokinin type B receptor Protein SEQUENCE
SEQUENCE 447 AA; 48419 MW; BAEEFAD4C1F85915 CRC64;
MELLKLNRSV QGTGPGPGAS LCRPGAPLLN SSSVGNLSCE PPRIRGAGTR
ELELAIRITL YAVIFLMSVG GNMLIIVVLG LSRRLRTVTN AFLLSLAVSD
LLLAVACMPF TLLPNLMGTF IFGTVICKAV SYLMGVSVSV STLSLVAIAL
ERYSAICRPL QARVWQTRSH AARVIVATWL LSGLLMVPYP VYTVVQPVGP
RVLQCVHRWP SARVRQTWSV LLLLLLFFIP GVVMAVAYGL ISRELYLGLR
FDGDSDSDSQ SRVRNQGGLP GAVHQNGRCR PETGAVGEDS DGCYVQLPRS
RPALELTALT APGPGSGSRP TQAKLLAKKR VVRMLLVIVV LFFLCWLPVY
SANTWRAFDG PGAHRALSGA PISFIHLLSY ASACVNPLVY CFMHRRFRQA
CLETCARCCP RPPRARPRAL PDEDPPTPSI ASLSRLSYTT ISTLGPG
UCSC uc001mcp.4; human. [P32239-1];

Category

Human CCKBR ELISA Kit