Human CHRNA1 ELISA Kit

Alternative name
Human ACHRA ELISA Kit;Human CHNRA ELISA Kit;Human ACHRD ELISA Kit;Human CHRNA ELISA Kit;Human CMS1A ELISA Kit;Human CMS1B ELISA Kit;Human CMS2A ELISA Kit;Human FCCMS ELISA Kit;Human SCCMS ELISA Kit;Human cholinergic receptor nicotinic alpha 1 subunit ELISA Kit;Human acetylcholine receptor subunit alpha ELISA Kit;Human acetylcholine receptor, nicotinic, alpha 1 (muscle) ELISA Kit;Human cholinergic receptor, nicotinic alpha 1 ELISA Kit;Human cholinergic receptor, nicotinic, alpha 1 (muscle) ELISA Kit;Human cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) ELISA Kit;Human muscle nicotinic acetylcholine receptor ELISA Kit;Human nicotinic acetylcholine receptor alpha subunit ELISA Kit;Human nicotinic cholinergic receptor alpha 1 ELISA Kit;
Catalog
E008275
species
Human
GeneCHRNA1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1). No significant cross-reactivity or interference between Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.29ng/mL.
Intended UseHuman CHRNA1 ELISA Kit allows for the in vitro quantitative determination of CHRNA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Acetylcholine receptor subunit alpha Protein information
Uniprot ID ACHA_HUMAN
Uniprot AC P02708; B4DRV6; D3DPE8;
UniGene Hs.434479;
GeneID 1134
KEGG hsa:1134;
Human Acetylcholine receptor subunit alpha Protein SEQUENCE
SEQUENCE 482 AA; 54546 MW; 8B307AD69B91A28B CRC64;
MEPWPLLLLF SLCSAGLVLG SEHETRLVAK LFKDYSSVVR PVEDHRQVVE
VTVGLQLIQL INVDEVNQIV TTNVRLKQGD MVDLPRPSCV TLGVPLFSHL
QNEQWVDYNL KWNPDDYGGV KKIHIPSEKI WRPDLVLYNN ADGDFAIVKF
TKVLLQYTGH ITWTPPAIFK SYCEIIVTHF PFDEQNCSMK LGTWTYDGSV
VAINPESDQP DLSNFMESGE WVIKESRGWK HSVTYSCCPD TPYLDITYHF
VMQRLPLYFI VNVIIPCLLF SFLTGLVFYL PTDSGEKMTL SISVLLSLTV
FLLVIVELIP STSSAVPLIG KYMLFTMVFV IASIIITVIV INTHHRSPST
HVMPNWVRKV FIDTIPNIMF FSTMKRPSRE KQDKKIFTED IDISDISGKP
GPPPMGFHSP LIKHPEVKSA IEGIKYIAET MKSDQESNNA AAEWKYVAMV
MDHILLGVFM LVCIIGTLAV FAGRLIELNQ QG
UCSC uc002ujd.3; human. [P02708-1];

Category

Human CHRNA1 ELISA Kit