Human CHRNA3 ELISA Kit

Alternative name
Human NACHRA3 ELISA Kit;Human LNCR2 ELISA Kit;Human PAOD2 ELISA Kit;Human cholinergic receptor nicotinic alpha 3 subunit ELISA Kit;Human neuronal acetylcholine receptor subunit alpha-3 ELISA Kit;Human cholinergic receptor, nicotinic alpha 3 ELISA Kit;Human cholinergic receptor, nicotinic, alpha 3 (neuronal) ELISA Kit;Human cholinergic receptor, nicotinic, alpha polypeptide 3 ELISA Kit;Human neuronal nicotinic acetylcholine receptor, alpha3 subunit ELISA Kit;
Catalog
E008281
species
Human
GeneCHRNA3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). No significant cross-reactivity or interference between Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.107ng/mL.
Intended UseHuman CHRNA3 ELISA Kit allows for the in vitro quantitative determination of CHRNA3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Neuronal acetylcholine receptor subunit alpha-3 Protein information
Uniprot ID ACHA3_HUMAN
Uniprot AC P32297; Q15823; Q4KMN8; Q86U77; Q96RH3; Q99553; Q9BQ93; Q9BRR4;
UniGene Hs.89605;
GeneID 1136
KEGG hsa:1136;
Human Neuronal acetylcholine receptor subunit alpha-3 Protein SEQUENCE
SEQUENCE 505 AA; 57480 MW; 478D7712D59ACB2D CRC64;
MGSGPLSLPL ALSPPRLLLL LLLSLLPVAR ASEAEHRLFE RLFEDYNEII
RPVANVSDPV IIHFEVSMSQ LVKVDEVNQI METNLWLKQI WNDYKLKWNP
SDYGGAEFMR VPAQKIWKPD IVLYNNAVGD FQVDDKTKAL LKYTGEVTWI
PPAIFKSSCK IDVTYFPFDY QNCTMKFGSW SYDKAKIDLV LIGSSMNLKD
YWESGEWAII KAPGYKHDIK YNCCEEIYPD ITYSLYIRRL PLFYTINLII
PCLLISFLTV LVFYLPSDCG EKVTLCISVL LSLTVFLLVI TETIPSTSLV
IPLIGEYLLF TMIFVTLSIV ITVFVLNVHY RTPTTHTMPS WVKTVFLNLL
PRVMFMTRPT SNEGNAQKPR PLYGAELSNL NCFSRAESKG CKEGYPCQDG
MCGYCHHRRI KISNFSANLT RSSSSESVDA VLSLSALSPE IKEAIQSVKY
IAENMKAQNE AKEIQDDWKY VAMVIDRIFL WVFTLVCILG TAGLFLQPLM
AREDA
UCSC uc002beb.4; human. [P32297-2];

Category

Human CHRNA3 ELISA Kit