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Human CHRNA7 ELISA Kit

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  • Alternative name

    Human NACHRA7 ELISA Kit;Human CHRNA7 ELISA Kit;Human CHRNA7-DR1 ELISA Kit;Human D-10 ELISA Kit;Human CHRNA7 (exons 5-10) and FAM7A (exons A-E) fusion ELISA Kit;Human CHRNA7-FAM7A fusion protein ELISA Kit;Human CHRNA7 (cholinergic receptor, nicotinic, alpha 7, exons 5-10) and FAM7A (family with sequence similarity 7A, exons A-E) fusion ELISA Kit;Human CHRNA7 (cholinergic receptor, nicotinic, alpha polypeptide 7, exons 5-10) and FAM7A (family with sequence similarity 7A, exons A-E) fusion ELISA Kit;Human alpha 7 neuronal nicotinic acetylcholine receptor-FAM7A hybrid ELISA Kit;Human alpha-7 nicotinic cholinergic receptor subunit ELISA Kit;
  • Catalog
    E008289
  • species
    Human
  • GeneCHRNA7
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cholinergic Receptor, Nicotinic, Alpha 7 (CHRNa7). No significant cross-reactivity or interference between Cholinergic Receptor, Nicotinic, Alpha 7 (CHRNa7) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.112ng/mL.
  • Intended UseHuman CHRNA7 ELISA Kit allows for the in vitro quantitative determination of CHRNA7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Alpha 7 (CHRNa7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Alpha 7 (CHRNa7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Nicotinic, Alpha 7 (CHRNa7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholinergic Receptor, Nicotinic, Alpha 7 (CHRNa7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Neuronal acetylcholine receptor subunit alpha-7 Protein information
  • Uniprot ID ACHA7_HUMAN
  • Uniprot AC P36544; A8K7Q4; B4DFS0; Q15826; Q8IUZ4; Q96RH2; Q99555; Q9BXH0;
  • UniGene Hs.510853; Hs.511772; Hs.713151;
  • GeneID 89832
  • KEGG hsa:1139;
  • Human Neuronal acetylcholine receptor subunit alpha-7 Protein SEQUENCE

  • SEQUENCE 502 AA; 56449 MW; D94B3A482EAA0E42 CRC64;

    MRCSPGGVWL ALAASLLHVS LQGEFQRKLY KELVKNYNPL ERPVANDSQP

    LTVYFSLSLL QIMDVDEKNQ VLTTNIWLQM SWTDHYLQWN VSEYPGVKTV

    RFPDGQIWKP DILLYNSADE RFDATFHTNV LVNSSGHCQY LPPGIFKSSC

    YIDVRWFPFD VQHCKLKFGS WSYGGWSLDL QMQEADISGY IPNGEWDLVG

    IPGKRSERFY ECCKEPYPDV TFTVTMRRRT LYYGLNLLIP CVLISALALL

    VFLLPADSGE KISLGITVLL SLTVFMLLVA EIMPATSDSV PLIAQYFAST

    MIIVGLSVVV TVIVLQYHHH DPDGGKMPKW TRVILLNWCA WFLRMKRPGE

    DKVRPACQHK QRRCSLASVE MSAVAPPPAS NGNLLYIGFR GLDGVHCVPT

    PDSGVVCGRM ACSPTHDEHL LHGGQPPEGD PDLAKILEEV RYIANRFRCQ

    DESEAVCSEW KFAACVVDRL CLMAFSVFTI ICTIGILMSA PNFVEAVSKD

    FA

  • UCSC uc001zft.5; human. [P36544-1];



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