Human CHRNB3 ELISA Kit

Alternative name
Human cholinergic receptor nicotinic beta 3 subunit ELISA Kit;Human neuronal acetylcholine receptor subunit beta-3 ELISA Kit;Human acetylcholine receptor, neuronal nicotinic, beta-3 subunit ELISA Kit;Human acetylcholine receptor, nicotinic, beta 3 (neuronal) ELISA Kit;Human cholinergic receptor, nicotinic beta 3 ELISA Kit;Human cholinergic receptor, nicotinic, beta 3 (neuronal) ELISA Kit;Human cholinergic receptor, nicotinic, beta polypeptide 3 ELISA Kit;
Catalog
E008294
species
Human
GeneCHRNB3
SpecificityThis assay has high sensitivity and excellent specificity for detection of CHRNb3. No significant cross-reactivity or interference between CHRNb3 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of CHRNb3 is typically less than 0.125ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding t
Intended UseHuman CHRNB3 ELISA Kit allows for the in vitro quantitative determination of CHRNB3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CHRNb3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CHRNb3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CHRNb3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CHRNb3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Neuronal acetylcholine receptor subunit beta-3 Protein information
Uniprot ID ACHB3_HUMAN
Uniprot AC Q05901; Q15827;
UniGene Hs.654576;
GeneID 1142
KEGG hsa:1142;
Human Neuronal acetylcholine receptor subunit beta-3 Protein SEQUENCE
SEQUENCE 458 AA; 52729 MW; D6E919E53CBD21F8 CRC64;
MLPDFMLVLI VLGIPSSATT GFNSIAENED ALLRHLFQGY QKWVRPVLHS
NDTIKVYFGL KISQLVDVDE KNQLMTTNVW LKQEWTDHKL RWNPDDYGGI
HSIKVPSESL WLPDIVLFEN ADGRFEGSLM TKVIVKSNGT VVWTPPASYK
SSCTMDVTFF PFDRQNCSMK FGSWTYDGTM VDLILINENV DRKDFFDNGE
WEILNAKGMK GNRRDGVYSY PFITYSFVLR RLPLFYTLFL IIPCLGLSFL
TVLVFYLPSD EGEKLSLSTS VLVSLTVFLL VIEEIIPSSS KVIPLIGEYL
LFIMIFVTLS IIVTVFVINV HHRSSSTYHP MAPWVKRLFL QKLPKLLCMK
DHVDRYSSPE KEESQPVVKG KVLEKKKQKQ LSDGEKVLVA FLEKAADSIR
YISRHVKKEH FISQVVQDWK FVAQVLDRIF LWLFLIVSVT GSVLIFTPAL
KMWLHSYH
UCSC uc003xpi.2; human.;

Category

Human CHRNB3 ELISA Kit