Human CHRFAM7A ELISA Kit

Alternative name
Human CHRNA7-DR1 ELISA Kit;Human D-10 ELISA Kit;Human CHRNA7 ELISA Kit;Human CHRNA7 (exons 5-10) and FAM7A (exons A-E) fusion ELISA Kit;Human CHRNA7-FAM7A fusion protein ELISA Kit;Human CHRNA7 (cholinergic receptor, nicotinic, alpha 7, exons 5-10) and FAM7A (family with sequence similarity 7A, exons A-E) fusion ELISA Kit;Human CHRNA7 (cholinergic receptor, nicotinic, alpha polypeptide 7, exons 5-10) and FAM7A (family with sequence similarity 7A, exons A-E) fusion ELISA Kit;Human alpha 7 neuronal nicotinic acetylcholine receptor-FAM7A hybrid ELISA Kit;Human alpha-7 nicotinic cholinergic receptor subunit ELISA Kit;
Catalog
E008366
species
Human
GeneCHRFAM7A
SpecificityThis assay has high sensitivity and excellent specificity for detection of CHRFAM7A. No significant cross-reactivity or interference between CHRFAM7A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CHRFAM7A and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CHRFAM7A ELISA Kit allows for the in vitro quantitative determination of CHRFAM7A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the assay: CHRFAM7A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CHRFAM7A antibody and an CHRFAM7A-HRP conjugate. The assay sample and buffer are incubated together with CHRFAM7A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHRFAM7A concentration since CHRFAM7A from samples and CHRFAM7A-HRP conjugate compete for the anti-CHRFAM7A antibody binding site. Since the number of sites is limited, as more sites are occupied by CHRFAM7A from the sample, fewer sites are left to bind CHRFAM7A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHRFAM7A concentration in each sample is interpolated from this standard curve.
Human CHRNA7-FAM7A fusion protein Protein information
Uniprot ID CRFM7_HUMAN
Uniprot AC Q494W8; A8KAB9;
UniGene Hs.510853;
GeneID 89832
KEGG hsa:89832;
Human CHRNA7-FAM7A fusion protein Protein SEQUENCE
SEQUENCE 412 AA; 46218 MW; 17D1A33E8540BF44 CRC64;
MQKYCIYQHF QFQLLIQHLW IAANCDIADE RFDATFHTNV LVNSSGHCQY
LPPGIFKSSC YIDVRWFPFD VQHCKLKFGS WSYGGWSLDL QMQEADISGY
IPNGEWDLVG IPGKRSERFY ECCKEPYPDV TFTVTMRRRT LYYGLNLLIP
CVLISALALL VFLLPADSGE KISLGITVLL SLTVFMLLVA EIMPATSDSV
PLIAQYFAST MIIVGLSVVV TVIVLQYHHH DPDGGKMPKW TRVILLNWCA
WFLRMKRPGE DKVRPACQHK QRRCSLASVE MSAVAPPPAS NGNLLYIGFR
GLDGVHCVPT PDSGVVCGRM ACSPTHDEHL LHGGQPPEGD PDLAKILEEV
RYIANRFRCQ DESEAVCSEW KFAACVVDRL CLMAFSVFTI ICTIGILMSA
PNFVEAVSKD FA
UCSC uc001zdt.2; human.;

Category

Human CHRFAM7A ELISA Kit