Human CHGA ELISA Kit

Alternative name
Human CGA ELISA Kit;Human pituitary secretory protein I ELISA Kit;Human SP-I ELISA Kit;Human Vasostatin I ELISA Kit;Human Vasostatin II ELISA Kit;Human SL21 ELISA Kit;Human chromogranin A ELISA Kit;Human chromogranin-A ELISA Kit;Human betagranin (N-terminal fragment of chromogranin A) ELISA Kit;Human catestatin ELISA Kit;Human chromofungin ELISA Kit;Human parathyroid secretory protein 1 ELISA Kit;
Catalog
E008407
species
Human
GeneCHGA
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman CHGA ELISA Kit allows for the in vitro quantitative determination of CHGA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the Assay: CHGA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CHGA antibody and an CHGA-HRP conjugate. The assay sample and buffer are incubated together with CHGA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHGA concentration since CHGA from samples and CHGA-HRP conjugate compete for the anti-CHGA antibody binding site. Since the number of sites is limited, as more sites are occupied by CHGA from the sample, fewer sites are left to bind CHGA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHGA concentration in each sample is interpolated from this standard curve.
Human p-Glu serpinin precursor Protein information
Uniprot ID CMGA_HUMAN
Uniprot AC P10645; B2R9E9; Q53FA8; Q6NR84; Q96E84; Q96GL7; Q9BQB5;
UniGene Hs.150793;
GeneID 1113
KEGG hsa:1113;
Human p-Glu serpinin precursor Protein SEQUENCE
SEQUENCE 457 AA; 50688 MW; 2F634E1A83FF0BB1 CRC64;
MRSAAVLALL LCAGQVTALP VNSPMNKGDT EVMKCIVEVI SDTLSKPSPM
PVSQECFETL RGDERILSIL RHQNLLKELQ DLALQGAKER AHQQKKHSGF
EDELSEVLEN QSSQAELKEA VEEPSSKDVM EKREDSKEAE KSGEATDGAR
PQALPEPMQE SKAEGNNQAP GEEEEEEEEA TNTHPPASLP SQKYPGPQAE
GDSEGLSQGL VDREKGLSAE PGWQAKREEE EEEEEEAEAG EEAVPEEEGP
TVVLNPHPSL GYKEIRKGES RSEALAVDGA GKPGAEEAQD PEGKGEQEHS
QQKEEEEEMA VVPQGLFRGG KSGELEQEEE RLSKEWEDSK RWSKMDQLAK
ELTAEKRLEG QEEEEDNRDS SMKLSFRARA YGFRGPGPQL RRGWRPSSRE
DSLEAGLPLQ VRGYPEEKKE EEGSANRRPE DQELESLSAI EAELEKVAHQ
LQALRRG
UCSC uc001ybc.6; human.;

Category

Human CHGA ELISA Kit