Human CKMT1B ELISA Kit

Alternative name
Human acidic-type mitochondrial creatine kinase ELISA Kit;Human mia-CK ELISA Kit;Human ubiquitous mitochondrial creatine kinase ELISA Kit;Human U-MtCK ELISA Kit;Human CKMT ELISA Kit;Human CKMT1 ELISA Kit;Human creatine kinase, mitochondrial 1A ELISA Kit;Human creatine kinase U-type, mitochondrial ELISA Kit;Human creatine kinase, mitochondrial 1 (ubiquitous) ELISA Kit;
Catalog
E009526
species
Human
GeneCKMT1B
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CKMT1B. No significant cross-reactivity or interference between Human CKMT1B and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CKMT1B and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.938ng/mL
Intended UseHuman CKMT1B ELISA Kit allows for the in vitro quantitative determination of CKMT1B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CKMT1B in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CKMT1B. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CKMT1B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CKMT1B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CKMT1B. You can calculate the concentration of Human CKMT1B in the samples by comparing the OD of the samples to the standard curve.
Human Creatine kinase U-type, mitochondrial Protein information
Uniprot ID KCRU_HUMAN
Uniprot AC P12532; B4DIT8; B7ZA09; Q0VAM3; Q32NF6; Q53FC4;
UniGene Hs.654988; Hs.741420;
GeneID 548596
KEGG hsa:548596;
Human Creatine kinase U-type, mitochondrial Protein SEQUENCE
SEQUENCE 417 AA; 47037 MW; 274DAC2E9A8AD882 CRC64;
MAGPFSRLLS ARPGLRLLAL AGAGSLAAGF LLRPEPVRAA SERRRLYPPS
AEYPDLRKHN NCMASHLTPA VYARLCDKTT PTGWTLDQCI QTGVDNPGHP
FIKTVGMVAG DEETYEVFAD LFDPVIQERH NGYDPRTMKH TTDLDASKIR
SGYFDERYVL SSRVRTGRSI RGLSLPPACT RAERREVERV VVDALSGLKG
DLAGRYYRLS EMTEAEQQQL IDDHFLFDKP VSPLLTAAGM ARDWPDARGI
WHNNEKSFLI WVNEEDHTRV ISMEKGGNMK RVFERFCRGL KEVERLIQER
GWEFMWNERL GYILTCPSNL GTGLRAGVHI KLPLLSKDSR FPKILENLRL
QKRGTGGVDT AATGGVFDIS NLDRLGKSEV ELVQLVIDGV NYLIDCERRL
ERGQDIRIPT PVIHTKH
UCSC uc001zsc.3; human. [P12532-1];

Category

Human CKMT1B ELISA Kit