Human CLDN18 ELISA Kit

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  • Alternative name

    Human SFTA5 ELISA Kit;Human SFTPJ ELISA Kit;Human claudin 18 ELISA Kit;Human claudin-18 ELISA Kit;Human surfactant associated 5 ELISA Kit;Human surfactant associated protein J ELISA Kit;Human surfactant, pulmonary associated protein J ELISA Kit;

  • Catalog
    E009582
  • species
    Human
  • GeneCLDN18
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Claudin 18 (CLDN18). No significant cross-reactivity or interference between Claudin 18 (CLDN18) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman CLDN18 ELISA Kit allows for the in vitro quantitative determination of CLDN18 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 18 (CLDN18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 18 (CLDN18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 18 (CLDN18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Claudin 18 (CLDN18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Claudin-18 Protein information
  • Uniprot ID CLD18_HUMAN
  • Uniprot AC P56856; A5PL21; Q96PH4;
  • UniGene Hs.655324;
  • GeneID 51208
  • KEGG hsa:51208;
  • Human Claudin-18 Protein SEQUENCE
  • SEQUENCE 261 AA; 27856 MW; 4362B590D3C2B387 CRC64;

    MSTTTCQVVA FLLSILGLAG CIAATGMDMW STQDLYDNPV TSVFQYEGLW

    RSCVRQSSGF TECRPYFTIL GLPAMLQAVR ALMIVGIVLG AIGLLVSIFA

    LKCIRIGSME DSAKANMTLT SGIMFIVSGL CAIAGVSVFA NMLVTNFWMS

    TANMYTGMGG MVQTVQTRYT FGAALFVGWV AGGLTLIGGV MMCIACRGLA

    PEETNYKAVS YHASGHSVAY KPGGFKASTG FGSNTKNKKI YDGGARTEDE

    VQSYPSKHDY V

  • UCSC uc003ero.2; human. [P56856-1];



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