Human CLDN5 ELISA Kit

Alternative name
Human transmembrane protein deleted in VCFS ELISA Kit;Human TMDVCF ELISA Kit;Human AWAL ELISA Kit;Human TMVCF ELISA Kit;Human BEC1 ELISA Kit;Human CPETRL1 ELISA Kit;Human claudin 5 ELISA Kit;Human claudin-5 ELISA Kit;Human transmembrane protein deleted in velocardiofacial syndrome ELISA Kit;
Catalog
E009606
species
Human
GeneCLDN5
SpecificityThis assay has high sensitivity and excellent specificity for detection of CLDN5. No significant cross-reactivity or interference between CLDN5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLDN5 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CLDN5 ELISA Kit allows for the in vitro quantitative determination of CLDN5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: CLDN5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLDN5 antibody and an CLDN5-HRP conjugate. The assay sample and buffer are incubated together with CLDN5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLDN5 concentration since CLDN5 from samples and CLDN5-HRP conjugate compete for the anti-CLDN5 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLDN5 from the sample, fewer sites are left to bind CLDN5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLDN5 concentration in each sample is interpolated from this standard curve.
Human Claudin-5 Protein information
Uniprot ID CLD5_HUMAN
Uniprot AC O00501; B3KS11; Q53XW2; Q8WUW3;
UniGene Hs.505337;
GeneID 7122
KEGG hsa:7122;
Human Claudin-5 Protein SEQUENCE
SEQUENCE 218 AA; 23147 MW; 9F7F9E194D25425F CRC64;
MGSAALEILG LVLCLVGWGG LILACGLPMW QVTAFLDHNI VTAQTTWKGL
WMSCVVQSTG HMQCKVYDSV LALSTEVQAA RALTVSAVLL AFVALFVTLA
GAQCTTCVAP GPAKARVALT GGVLYLFCGL LALVPLCWFA NIVVREFYDP
SVPVSQKYEL GAALYIGWAA TALLMVGGCL LCCGAWVCTG RPDLSFPVKY
SAPRRPTATG DYDKKNYV
UCSC uc062bmp.1; human.;

Category

Human CLDN5 ELISA Kit

Human transmembrane protein deleted in VCFS ELISA Kit;Human TMDVCF ELISA Kit;Human AWAL ELISA Kit;Human TMVCF ELISA Kit;Human BEC1 ELISA Kit;Human CPETRL1 ELISA Kit;Human claudin 5 ELISA Kit;Human claudin-5 ELISA Kit;Human transmembrane protein deleted in velocardiofacial syndrome ELISA Kit;