Human CD4 ELISA Kit

Alternative name
Human T-cell surface antigen T4/Leu-3 ELISA Kit;Human CD4mut ELISA Kit;Human CD4 molecule ELISA Kit;Human T-cell surface glycoprotein CD4 ELISA Kit;Human CD4 antigen (p55) ELISA Kit;Human CD4 receptor ELISA Kit;
Catalog
E009701
species
Human
GeneCD4
SpecificityThis assay has high sensitivity and excellent specificity for detection of CD4. No significant cross-reactivity or interference between CD4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD4 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman CD4 ELISA Kit allows for the in vitro quantitative determination of CD4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the assay: CD4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CD4. Standards or samples are then added to the microtiter plate wells and CD4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CD4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CD4 are added to each well to "sandwich" the CD4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CD4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD4 concentration in each sample is interpolated from this standard curve.
Human T-cell surface glycoprotein CD4 Protein information
Uniprot ID CD4_HUMAN
Uniprot AC P01730; B2R737; D3DUS5; Q4ZGK2; Q5U066; Q9UDE5;
UniGene Hs.631659;
GeneID 920
KEGG hsa:920;
Human T-cell surface glycoprotein CD4 Protein SEQUENCE
SEQUENCE 458 AA; 51111 MW; 20ED893F9E56D236 CRC64;
MNRGVPFRHL LLVLQLALLP AATQGKKVVL GKKGDTVELT CTASQKKSIQ
FHWKNSNQIK ILGNQGSFLT KGPSKLNDRA DSRRSLWDQG NFPLIIKNLK
IEDSDTYICE VEDQKEEVQL LVFGLTANSD THLLQGQSLT LTLESPPGSS
PSVQCRSPRG KNIQGGKTLS VSQLELQDSG TWTCTVLQNQ KKVEFKIDIV
VLAFQKASSI VYKKEGEQVE FSFPLAFTVE KLTGSGELWW QAERASSSKS
WITFDLKNKE VSVKRVTQDP KLQMGKKLPL HLTLPQALPQ YAGSGNLTLA
LEAKTGKLHQ EVNLVVMRAT QLQKNLTCEV WGPTSPKLML SLKLENKEAK
VSKREKAVWV LNPEAGMWQC LLSDSGQVLL ESNIKVLPTW STPVQPMALI
VLGGVAGLLL FIGLGIFFCV RCRHRRRQAE RMSQIKRLLS EKKTCQCPHR
FQKTCSPI
UCSC uc001qqv.3; human.;

Category

Human CD4 ELISA Kit

Human T-cell surface antigen T4/Leu-3 ELISA Kit;Human CD4mut ELISA Kit;Human CD4 molecule ELISA Kit;Human T-cell surface glycoprotein CD4 ELISA Kit;Human CD4 antigen (p55) ELISA Kit;Human CD4 receptor ELISA Kit;