Human CD5 ELISA Kit

Alternative name
Human lymphocyte antigen T1/Leu-1 ELISA Kit;Human LEU1 ELISA Kit;Human T1 ELISA Kit;Human CD5 molecule ELISA Kit;Human T-cell surface glycoprotein CD5 ELISA Kit;Human CD5 antigen (p56-62) ELISA Kit;
Catalog
E009715
species
Human
GeneCD5
SpecificityThis assay has high sensitivity and excellent specificity for detection of D5. No significant cross-reactivity or interference between D5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between D5 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CD5 ELISA Kit allows for the in vitro quantitative determination of CD5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the assay: D5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-D5 antibody and an D5-HRP conjugate. The assay sample and buffer are incubated together with D5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the D5 concentration since D5 from samples and D5-HRP conjugate compete for the anti-D5 antibody binding site. Since the number of sites is limited, as more sites are occupied by D5 from the sample, fewer sites are left to bind D5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The D5 concentration in each sample is interpolated from this standard curve.
Human T-cell surface glycoprotein CD5 Protein information
Uniprot ID CD5_HUMAN
Uniprot AC P06127; A0N0P4; A8K9I3;
UniGene Hs.58685;
GeneID 921
KEGG hsa:921;
Human T-cell surface glycoprotein CD5 Protein SEQUENCE
SEQUENCE 495 AA; 54578 MW; 9131AEC9683EE1D3 CRC64;
MPMGSLQPLA TLYLLGMLVA SCLGRLSWYD PDFQARLTRS NSKCQGQLEV
YLKDGWHMVC SQSWGRSSKQ WEDPSQASKV CQRLNCGVPL SLGPFLVTYT
PQSSIICYGQ LGSFSNCSHS RNDMCHSLGL TCLEPQKTTP PTTRPPPTTT
PEPTAPPRLQ LVAQSGGQHC AGVVEFYSGS LGGTISYEAQ DKTQDLENFL
CNNLQCGSFL KHLPETEAGR AQDPGEPREH QPLPIQWKIQ NSSCTSLEHC
FRKIKPQKSG RVLALLCSGF QPKVQSRLVG GSSICEGTVE VRQGAQWAAL
CDSSSARSSL RWEEVCREQQ CGSVNSYRVL DAGDPTSRGL FCPHQKLSQC
HELWERNSYC KKVFVTCQDP NPAGLAAGTV ASIILALVLL VVLLVVCGPL
AYKKLVKKFR QKKQRQWIGP TGMNQNMSFH RNHTATVRSH AENPTASHVD
NEYSQPPRNS HLSAYPALEG ALHRSSMQPD NSSDSDYDLH GAQRL
UCSC uc009ynk.4; human.;

Category

Human CD5 ELISA Kit

Human lymphocyte antigen T1/Leu-1 ELISA Kit;Human LEU1 ELISA Kit;Human T1 ELISA Kit;Human CD5 molecule ELISA Kit;Human T-cell surface glycoprotein CD5 ELISA Kit;Human CD5 antigen (p56-62) ELISA Kit;