Human CD74 ELISA Kit

Alternative name
Human HLA-DR antigens-associated invariant chain ELISA Kit;Human Ia antigen-associated invariant chain ELISA Kit;Human II ELISA Kit;Human p33 ELISA Kit;Human DHLAG ELISA Kit;Human HLADG ELISA Kit;Human Ia-GAMMA ELISA Kit;Human CD74 molecule ELISA Kit;Human HLA class II histocompatibility antigen gamma chain ELISA Kit;Human CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) ELISA Kit;Human CD74 molecule, major histocompatibility complex, class II invariant chain ELISA Kit;Human HLA-DR-gamma ELISA Kit;Human Ia-associated invariant chain ELISA Kit;Human MHC HLA-DR gamma chain ELISA Kit;Human gamma chain of class II antigens ELISA Kit;
Catalog
E009726
species
Human
GeneCD74
SpecificityThis assay has high sensitivity and excellent specificity for detection of CD74. No significant cross-reactivity or interference between CD74 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD74 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CD74 ELISA Kit allows for the in vitro quantitative determination of CD74 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the assay: CD74 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD74 antibody and an CD74-HRP conjugate. The assay sample and buffer are incubated together with CD74-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD74 concentration since CD74 from samples and CD74-HRP conjugate compete for the anti-CD74 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD74 from the sample, fewer sites are left to bind CD74-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD74 concentration in each sample is interpolated from this standard curve.
Human HLA class II histocompatibility antigen gamma chain Protein information
Uniprot ID HG2A_HUMAN
Uniprot AC Q8SNA0; Q8WLP6;
UniGene Hs.436568;
GeneID 972
KEGG hsa:972;
Human HLA class II histocompatibility antigen gamma chain Protein SEQUENCE
SEQUENCE 296 AA; 33516 MW; 27A13F252D5FB91D CRC64;
MHRRRSRSCR EDQKPVMDDQ RDLISNNEQL PMLGRRPGAP ESKCSRGALY
TGFSILVTLL LAGQATTAYF LYQQQGRLDK LTVTSQNLQL ENLRMKLPKP
PKPVSKMRMA TPLLMQALPM GALPQGPMQN ATKYGNMTED HVMHLLQNAD
PLKVYPPLKG SFPENLRHLK NTMETIDWKV FESWMHHWLL FEMSRHSLEQ
KPTDAPPKVL TKCQEEVSHI PAVHPGSFRP KCDENGNYLP LQCYGSIGYC
WCVFPNGTEV PNTRSRGHHN CSESLELEDP SSGLGVTKQD LGPVPM
UCSC uc003lsc.4; human. [P04233-1];

Category

Human CD74 ELISA Kit