Human F2 ELISA Kit

Alternative name
Human Coagulation factor II ELISA Kit;Human PT ELISA Kit;Human RPRGL2 ELISA Kit;Human THPH1 ELISA Kit;Human coagulation factor II, thrombin ELISA Kit;Human prothrombin ELISA Kit;Human prepro-coagulation factor II ELISA Kit;Human prothrombin B-chain ELISA Kit;Human serine protease ELISA Kit;
Catalog
E009806
species
Human
GeneF2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Coagulation Factor II (F2). No significant cross-reactivity or interference between Coagulation Factor II (F2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.17ng/mL.
Intended UseHuman F2 ELISA Kit allows for the in vitro quantitative determination of F2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor II (F2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor II (F2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor II (F2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor II (F2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Thrombin heavy chain Protein information
Uniprot ID THRB_HUMAN
Uniprot AC Q9UCA1;
UniGene Hs.655207;
GeneID 2147
KEGG hsa:2147;
Human Thrombin heavy chain Protein SEQUENCE
SEQUENCE 622 AA; 70037 MW; 8A25E1DA88208FCF CRC64;
MAHVRGLQLP GCLALAALCS LVHSQHVFLA PQQARSLLQR VRRANTFLEE
VRKGNLEREC VEETCSYEEA FEALESSTAT DVFWAKYTAC ETARTPRDKL
AACLEGNCAE GLGTNYRGHV NITRSGIECQ LWRSRYPHKP EINSTTHPGA
DLQENFCRNP DSSTTGPWCY TTDPTVRRQE CSIPVCGQDQ VTVAMTPRSE
GSSVNLSPPL EQCVPDRGQQ YQGRLAVTTH GLPCLAWASA QAKALSKHQD
FNSAVQLVEN FCRNPDGDEE GVWCYVAGKP GDFGYCDLNY CEEAVEEETG
DGLDEDSDRA IEGRTATSEY QTFFNPRTFG SGEADCGLRP LFEKKSLEDK
TERELLESYI DGRIVEGSDA EIGMSPWQVM LFRKSPQELL CGASLISDRW
VLTAAHCLLY PPWDKNFTEN DLLVRIGKHS RTRYERNIEK ISMLEKIYIH
PRYNWRENLD RDIALMKLKK PVAFSDYIHP VCLPDRETAA SLLQAGYKGR
VTGWGNLKET WTANVGKGQP SVLQVVNLPI VERPVCKDST RIRITDNMFC
AGYKPDEGKR GDACEGDSGG PFVMKSPFNN RWYQMGIVSW GEGCDRDGKY
GFYTHVFRLK KWIQKVIDQF GE
UCSC uc001ndf.5; human.;

Category

Human F2 ELISA Kit

Human Coagulation factor II ELISA Kit;Human PT ELISA Kit;Human RPRGL2 ELISA Kit;Human THPH1 ELISA Kit;Human coagulation factor II, thrombin ELISA Kit;Human prothrombin ELISA Kit;Human prepro-coagulation factor II ELISA Kit;Human prothrombin B-chain ELISA Kit;Human serine protease ELISA Kit;