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Alternative name
HEXC, KIAA0679, MEA5 antibody
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Catalog
A005164
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityMonoclonal Antibody
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Host Mouse
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Species ReactivityHuman
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Applications Tested/SuitableELISA,WB,IF,IHC
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PurificationProtein A+G purification
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Immunogenmeningioma expressed antigen 5 (hyaluronidase)
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IHCImmunohistochemistry of paraffin-embedded human gliomas using A005164(MGEA5 antibody) at dilution of 1:50
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Western BlotHuman brain lysate were subjected to SDS PAGE followed by western blot with A005164(MGEA5 antibody) at dilution of 1:600
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Recommended dilutionWB : 1:500-1:5000
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Product Description specificalIsoform 1: Cleaves GlcNAc but not GalNAc from O- glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4- methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl- beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714). _x000D_Isoform 3: Cleaves GlcNAc but not GalNAc from O- glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl- alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
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Gene nameMGEA5
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Uniprot IDO60502
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Observed M.W.130 kDa
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