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Alternative name
PCNA antibody
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Catalog
A006217
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityMonoclonal Antibody
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Host Mouse
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Species ReactivityHuman, Mouse
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Applications Tested/SuitableELISA,WB,IHC,FC,IP
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PurificationProtein A+G purification
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Immunogenproliferating cell nuclear antigen
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IHCImmunohistochemistry of paraffin-embedded human breast cancer using A006217(PCNA antibody) at dilution of 1:800
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Western BlotHEK-293 cells were subjected to SDS PAGE followed by western blot with A006217( PCNA Antibody) at various dilution.
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Recommended dilutionWB : 1:1000-1:10000
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Product Description specificalAuxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
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Gene namePCNA
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Uniprot IDP12004
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Observed M.W.36-38 kDa
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