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Alternative name
PMSL2 antibody
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Catalog
A006579
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityMonoclonal Antibody
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Host Mouse
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Species ReactivityHuman
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Applications Tested/SuitableELISA,WB,IHC
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PurificationProtein A+G purification
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ImmunogenPMS2 postmeiotic segregation increased 2 (S. cerevisiae)
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IHCImmunohistochemistry of paraffin-embedded human cervical cancer tissue slide using A006579( PMS2 Antibody) at dilution of 1:400
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Western BlotA431 cells were subjected to SDS PAGE followed by western blot with A006579(PMS2 antibody) at dilution of 1:1000
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Recommended dilutionWB : 1:200-1:2000
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Product Description specificalComponent of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2- MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.
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Gene namePMS2
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Uniprot IDP54278
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Observed M.W.117 kDa,100 kDa
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