-
Alternative name
ATP5A, ATP5AL2, ATPM antibody
-
Catalog
A000703
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityMonoclonal Antibody
-
Host Mouse
-
Species ReactivityHuman, Mouse ,Rat, Monkey
-
Applications Tested/SuitableELISA,IHC,IF,WB,FC
-
PurificationProtein A+G purification
-
ImmunogenATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
-
IHCImmunohistochemistry of paraffin-embedded human heart using A000703(ATP5A1 antibody) at dilution of 1:50
-
Western BlotWestern blot analysis of ATP5A1 in various tissues and cell lines using Fine Test antibody A000703
-
Recommended dilutionWB : 1:500-1:5000
-
Product Description specificalMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).
-
Gene nameATP5A1
-
Uniprot IDP25705
-
Observed M.W.50-55 kDa
-