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Alternative name
ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, β actin antibody
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Catalog
A000870
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityMonoclonal Antibody
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Host Mouse
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Species ReactivityHuman, Mouse ,Rat,Zebrafish,Pig,yeast
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Applications Tested/SuitableELISA,IHC,WB,IF,FC
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PurificationProtein A+G purification
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Immunogenactin, beta
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IHCImmunohistochemistry of paraffin-embedded human heart slide using A000870( beta actin Antibody) at dilution of 1:50
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Western BlotA549 cells were subjected to SDS PAGE followed by western blot with A000870(ACTB antibody) at dilution of 1:2000
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Recommended dilutionWB:1:2000-1:20000;IHC:1:50-1:500;IF:1:20-1:200
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Product Description specificalBeta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins.
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Gene nameACTB
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Uniprot IDP60709
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Calculated M.W.42kDa
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Observed M.W.42 kDa
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