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Alternative name
Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNFSF2, Tumor necrosis factor antibody
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Catalog
A008815
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityMonoclonal Antibody
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Host Mouse
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Species ReactivityHuman, Mouse
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Applications Tested/SuitableELISA,WB,IHC
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PurificationProtein A+G purification
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Immunogentumor necrosis factor (TNF superfamily, member 2)
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IHCImmunohistochemistry of paraffin-embedded human breast cancer tissue slide using A008815( TNF-a Antibody) at dilution of 1:800. heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Western BlotLPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with A008815( TNF-a Antibody) at dilution of 1:1000
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Recommended dilutionWB : 1:500-1:5000
IHC : 1:200-1:1000
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Product Description specificalTNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
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Gene nameTNF
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Uniprot IDP01375
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Calculated M.W.22kDa
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Observed M.W.17-22 kDa
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