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Alternative name
DJ447F3.2, UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C antibody
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Catalog
A009166
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityMonoclonal Antibody
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Host Mouse
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Species ReactivityHuman
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Applications Tested/SuitableELISA,IF,IHC,WB
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PurificationProtein A+G purification
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Immunogenubiquitin-conjugating enzyme E2C
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IHCImmunohistochemistry of paraffin-embedded human colon cancer slide using A009166( UBE2C Antibody) at dilution of 1:50
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Western BlotHEK-293 cells were subjected to SDS PAGE followed by western blot with A009166( UBE2C Antibody) at dilution of 1:1000
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Recommended dilutionWB : 1:500-1:5000;IHC:1:20-1:200；IF:1:10-1:100
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Product Description specificalAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
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Gene nameUBE2C
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locationCytoplasm,uclear
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Uniprot IDO00762
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Calculated M.W.20kDa
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Observed M.W.20 kDa
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