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Alternative name
FLJ36605, VIM, Vimentin antibody
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Catalog
A009410
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityMonoclonal Antibody
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Host Mouse
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Species ReactivityHuman
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Applications Tested/SuitableELISA,WB
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PurificationProtein A+G purification
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Immunogenvimentin
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IHCImmunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using A009410( Vimentin Antibody) at dilution of 1:500.heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Western BlotJurkat cells were subjected to SDS PAGE followed by western blot with A009410( Vimentin Antibody) at dilution of 1:2000
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Recommended dilutionWB :1:1000-1:10000;IHC:1:20-1:2000;IF:1:20-1:200
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Product Description specificalVimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
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Gene nameVIM
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locationCytoplasm,Intermediate filament
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Uniprot IDP08670
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Calculated M.W.55kDa
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Observed M.W.57 kDa
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