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Alternative name
RNF56 antibody
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Catalog
A001318
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IF
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PurificationImmunogen affinity purified
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ImmunogenCas-Br-M (murine) ecotropic retroviral transforming sequence b
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IHCImmunohistochemistry of paraffin-embedded human lung cancer using A001318(CBLB antibody) at dilution of 1:100
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Western BlotHEK-293 cells were subjected to SDS PAGE followed by western blot with A001318(CBLB antibody) at dilution of 1:500
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Recommended dilutionWB : 1:500-1:5000
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Product Description specificalE3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B- cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T- cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.
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Gene nameCBLB
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Uniprot IDQ13191
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Observed M.W.109 kDa
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