-
Alternative name
ADAR, ADAR1, adenosine deaminase, RNA, DRADA, DSH, DSRAD, G1P1, IFI 4, IFI4, Interferon inducible protein 4, K88dsRBP, p136 antibody
-
Catalog
A000150
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityPolyclonal Antibody
-
Host Rabbit
-
Species ReactivityHuman,Mouse ,Rat,Pig
-
Applications Tested/SuitableELISA,IHC,IF,WB,IP
-
PurificationImmunogen affinity purified
-
Immunogenadenosine deaminase, RNA-specific
-
IHCImmunohistochemistry of paraffin-embedded human gliomas using A000150(ADAR1 antibody) at dilution of 1:50
-
Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A000150( ADAR1 Antibody) at dilution of 1:1000
-
Recommended dilutionWB :1:500-1:5000;IHC:1:20-1:200;IP:1:200-1:2000;IF:1:20-1:200
-
Product Description specificalADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region(PMID:15556947). The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an interferon (IFN) inducible ?150 kDa protein and a constitutively expressed N-terminally truncated ?110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters(PMID:11111054). It has 5 isoforms produced by alternative promoter usage and alternative splicing. Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH).ADAR1 can form respective homodimers, and this association is essential for its enzymatic activities(PMID:17428802).
-
Gene nameADAR
-
locationNucleus,ytoplasm
-
Uniprot IDP55265
-
Calculated M.W.136kDa,observed 110kDa
-
Observed M.W.110 kDa
-