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Alternative name
CDK8, Cell division protein kinase 8, cyclin dependent kinase 8, K35, Mediator complex subunit CDK8, Protein kinase K35 antibody
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Catalog
A001563
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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Immunogencyclin-dependent kinase 8
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IHCImmunohistochemistry of paraffin-embedded human stomach cancer using A001563(CDK8 antibody) at dilution of 1:50
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Western BlotNIH/3T3 cells were subjected to SDS PAGE followed by western blot with A001563(CDK8 antibody) at dilution of 1:500
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Recommended dilutionWB : 1:500-1:5000
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Product Description specificalComponent of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
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Gene nameCDK8
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Uniprot IDP49336
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Observed M.W.53 kDa
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