-
Alternative name
MIA40 antibody
-
Catalog
A001637
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityPolyclonal Antibody
-
Host Rabbit
-
Species ReactivityHuman,Mouse ,Rat
-
Applications Tested/SuitableELISA,IHC,IF,WB,IP
-
PurificationImmunogen affinity purified
-
Immunogencoiled-coil-helix-coiled-coil-helix domain containing 4
-
IHCImmunohistochemistry of paraffin-embedded human kidney using A001637(CHCHD4 antibody) at dilution of 1:100
-
Western Blotrat brain tissue were subjected to SDS PAGE followed by western blot with A001637( CHCHD4 Antibody) at dilution of 1:1500
-
Recommended dilutionWB : 1:500-1:5000
-
Product Description specificalFunctions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17 or MICU1 (PubMed:16185709, PubMed:26387864, PubMed:19182799, PubMed:21059946, PubMed:23186364). Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS) (PubMed:16185709, PubMed:19182799, PubMed:21059946). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria (PubMed:16185709, PubMed:19182799, PubMed:21059946). The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS (PubMed:16185709, PubMed:19182799, PubMed:21059946). Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system (PubMed:23186364). Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake (PubMed:26387864).
-
Gene nameCHCHD4
-
Uniprot IDQ8N4Q1
-
Observed M.W.22-25 kDa
-