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Alternative name
AGAP 2, AGAP2, Centaurin gamma 1, CENTG1, Cnt g1, FLJ16430, GGAP2, KIAA0167, PIKE antibody
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Catalog
A000205
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IF
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PurificationImmunogen affinity purified
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ImmunogenArfGAP with GTPase domain, ankyrin repeat and PH domain 2
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IHCImmunohistochemistry of paraffin-embedded human gliomas using A000205(AGAP2 antibody) at dilution of 1:100
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Western Blothuman brain tissue were subjected to SDS PAGE followed by western blot with A000205(AGAP2 antibody) at dilution of 1:500
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Recommended dilutionWB :1:500-1:5000;IP:1:200-1:2000;IHC:1:20-1:200;IF:1:20-1:200
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Product Description specificalGTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
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Gene nameAGAP2
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locationCytoplasm,Nucleus
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Uniprot IDQ99490
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Calculated M.W.125kDa
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Observed M.W.124 kDa
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