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Alternative name
DP103, GEMIN3 antibody
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Catalog
A002298
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IF,IP,FC
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PurificationImmunogen affinity purified
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ImmunogenDEAD (Asp-Glu-Ala-Asp) box polypeptide 20
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IHCImmunohistochemistry of paraffin-embedded human breast cancer tissue slide using A002298( DDX20 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A002298(DDX20 antibody) at dilution of 1:500
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Recommended dilutionWB : 1:1000-1:10000
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Product Description specificalThe SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).
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Gene nameDDX20
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Uniprot IDQ9UHI6
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Observed M.W.100 kDaa; 112-115 kDa
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