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Alternative name
AIF, AIFM1, PDCD8 antibody
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Catalog
A000235
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,IHC,WB,IP,IF
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PurificationImmunogen affinity purified
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Immunogenapoptosis-inducing factor, mitochondrion-associated, 1
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IHCImmunohistochemistry of paraffin-embedded human kidney tissue slide using A000235( AIF Antibody) at dilution of 1:200
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Western BlotJurkat cells were subjected to SDS PAGE followed by western blot with A000235(AIF antibody) at dilution of 1:2000
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Recommended dilutionWB :1:1000-1:10000;IP:1:1000-1:10000;IF:1:10-1:100;IHC:1:100-400
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Product Description specificalApoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).
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Gene nameAIFM1
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locationCytoplasm,Membrane,Mitochondrion,Mitochondrion inner membrane,Nucleus
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Uniprot IDO95831
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Calculated M.W.66kDa
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Observed M.W.67 kDa
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