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Alternative name
ERBB, ERBB1, HER1 antibody
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Catalog
A002668
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman
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Applications Tested/SuitableELISA,WB,IHC,IP,IF
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PurificationImmunogen affinity purified
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Immunogenepidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
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IHCImmunofluorescent analysis of Hela cells, using A002668( EGFR antibody ) at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Western BlotA431 cells were subjected to SDS PAGE followed by western blot with A002668( EGFR-Specific Antibody) at dilution of 1:1000
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Product Description specificalReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin._x000D_Isoform 2 may act as an antagonist of EGF action.
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Uniprot IDP00533
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