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Alternative name
AKT3, DKFZp434N0250, PKB GAMMA, PKBG, PRKBG, Protein kinase Akt 3, Protein kinase B gamma, RAC gamma, RAC PK gamma, STK 2 antibody
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Catalog
A000273
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat,Zebrafish
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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Immunogenv-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
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IHCImmunohistochemistry of paraffin-embedded human kidney tissue slide using A000273( AKT3 Antibody) at dilution of 1:50
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Western BlotMCF7 cells were subjected to SDS PAGE followed by western blot with A000273(AKT3 antibody) at dilution of 1:500
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Recommended dilutionWB :1:200-1:2000;IHC:1:20-1:200
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Product Description specificalAKT3 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase- dependent apoptosis.
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Gene nameAKT3
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locationCytoplasm,Membrane,Nucleus
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Uniprot IDQ9Y243
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Calculated M.W.54kDa
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Observed M.W.60kd
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