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Alternative name
Gi, GNAI1 antibody
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Catalog
A003531
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,FC,IF
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PurificationImmunogen affinity purified
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Immunogenguanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
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IHCImmunohistochemistry of paraffin-embedded human gliomas using A003531(GNAI1 antibody) at dilution of 1:100
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Western Blothuman brain tissue were subjected to SDS PAGE followed by western blot with A003531(GNAI1 antibody) at dilution of 1:1600
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Recommended dilutionWB : 1:500-1:5000
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Product Description specificalGuanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (By similarity). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis (PubMed:17635935).
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Gene nameGNAI1
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Uniprot IDP63096
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Observed M.W.40 kDa
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