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Alternative name
87U6, FLJ26559, G(i) alpha 3, GNAI3 antibody
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Catalog
A003533
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IF
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PurificationImmunogen affinity purified
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Immunogenguanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
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IHCImmunohistochemistry of paraffin-embedded human kidney using A003533(GNAI3 antibody) at dilution of 1:50
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Western Blotmouse kidney tissue were subjected to SDS PAGE followed by western blot with A003533(GNAI3 antibody) at dilution of 1:500
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Recommended dilutionWB : 1:200-1:2000
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Product Description specificalHeterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541, PubMed:19478087). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087). Stimulates the activity of receptor-regulated K(+) channels (PubMed:2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935).
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Gene nameGNAI3
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Uniprot IDP08754
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Observed M.W.41-45 kDa
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