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Alternative name
Amphiregulin, AR, AREG, AREGB, CRDGF, SDGF antibody
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Catalog
A000374
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman
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Applications Tested/SuitableELISA,IF,WB,IHC,FC,IP
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PurificationImmunogen affinity purified
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Immunogenamphiregulin
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IHCImmunohistochemistry of paraffin-embedded human colon cancer tissue slide using A000374( AREG Antibody) at dilution of 1:200. heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Western BlotMCF-7 cells were subjected to SDS PAGE followed by western blot with A000374( AREG Antibody) at dilution of 1:600
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Recommended dilutionWB :1:200-1:2000;IHC:1:50-1:500;IP:1:1000-1:10000;IF:1:10-1:100
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Product Description specificalAmphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms (PMID: 9642297).
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Gene nameAREG
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locationMembrane
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Uniprot IDP15514
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Calculated M.W.28kDa,observed 43kDa
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Observed M.W.43 kDa
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