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Alternative name
AUF1, HNRPD antibody
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Catalog
A003950
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IF
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PurificationImmunogen affinity purified
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Immunogenheterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
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IHCImmunohistochemistry of paraffin-embedded human cervical cancer using A003950(HNRNPD antibody) at dilution of 1:100
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Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A003950(HNRNPD antibody) at dilution of 1:500
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Recommended dilutionWB : 1:500-1:5000
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Product Description specificalBinds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto- oncogenes and cytokine mRNAs. Also binds to double- and single- stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'- TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. May play a role in the regulation of the rhythmic expression of circadian clock core genes. Directly binds to the 3'UTR of CRY1 mRNA and induces CRY1 rhythmic translation. May also be involved in the regulation of PER2 translation.
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Gene nameHNRNPD
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Uniprot IDQ14103
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Observed M.W.38 kDa and 40 kDa
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