-
Alternative name
HEP, HPA, HPA1, HPR1, HPSE1, HSE1 antibody
-
Catalog
A004003
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityPolyclonal Antibody
-
Host Rabbit
-
Species ReactivityHuman,Mouse ,Rat
-
Applications Tested/SuitableELISA,IHC,WB
-
PurificationImmunogen affinity purified
-
Immunogenheparanase
-
IHCImmunohistochemistry of paraffin-embedded human liver cancer using A004003(HPSE antibody) at dilution of 1:50
-
Western BlotDU 145 cells were subjected to SDS PAGE followed by western blot with A004003(HPSE antibody) at dilution of 1:300
-
Recommended dilutionWB:1:200-1:1000;IHC:1:20-1:200
-
Product Description specificalEndoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up- regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.
-
Gene nameHPSE
-
Uniprot IDQ9Y251
-
Calculated M.W.60kDa,observed 50kDa,65kDa
-
Observed M.W.60 kDa
-