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Alternative name
interferon regulatory factor 3, IRF 3, IRF3 antibody
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Catalog
A004389
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IF,IP,FC
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PurificationImmunogen affinity purified
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Immunogeninterferon regulatory factor 3
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IHCImmunohistochemistry of paraffin-embedded human spleen using A004389(IRF3 antibody) at dilution of 1:50
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Western BlotJurkat cells were subjected to SDS PAGE followed by western blot with A004389(IRF3 antibody) at dilution of 1:1000
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Recommended dilutionWB : 1:500-1:5000
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Product Description specificalKey transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
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Gene nameIRF3
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Uniprot IDQ14653
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Observed M.W.49-55 kDa
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