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Alternative name
MNPEP, P67EIF2 antibody
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Catalog
A005135
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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Immunogenmethionyl aminopeptidase 2
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IHCImmunohistochemistry of paraffin-embedded human testis using A005135(METAP2 antibody) at dilution of 1:50
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Western BlotHepG2 cells were subjected to SDS PAGE followed by western blot with A005135(METAP2 antibody) at dilution of 1:300
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Recommended dilutionWB : 1:200-1:2000
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Product Description specificalCotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N- terminal Met-Val and Met-Thr sequences in vivo._x000D_Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
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Gene nameMETAP2
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Uniprot IDP50579
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Observed M.W.67 kDa
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