-
Alternative name
ADP ribosylation factor 1, ARF1 antibody
-
Catalog
A000528
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityPolyclonal Antibody
-
Host Rabbit
-
Species ReactivityHuman,Mouse ,Rat, Monkey
-
Applications Tested/SuitableELISA,WB,IHC
-
PurificationImmunogen affinity purified
-
ImmunogenADP-ribosylation factor 1
-
IHCImmunohistochemistry of paraffin-embedded human small intestine tissue slide using A000528( ARF1 Antibody) at dilution of 1:50
-
Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A000528(ARF1 antibody) at dilution of 1:500
-
Recommended dilutionWB : 1:200-1:2000
-
Product Description specificalGTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).
-
Gene nameARF1
-
Uniprot IDP84077
-
Observed M.W.21 kDa
-