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Alternative name
TIM antibody
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Catalog
A000563
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IP
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PurificationImmunogen affinity purified
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ImmunogenRho guanine nucleotide exchange factor (GEF) 5
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IHCImmunohistochemistry of paraffin-embedded human lung cancer tissue slide using A000563( ARHGEF5 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Western BlotMCF7 cells were subjected to SDS PAGE followed by western blot with A000563(ARHGEF5 antibody) at dilution of 1:500
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Recommended dilutionWB : 1:1000-1:10000
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Product Description specificalGuanine nucleotide exchange factor which activates Rho GTPases (PubMed:15601624). Strongly activates RHOA (PubMed:15601624). Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (By similarity). Involved in regulation of cell shape and actin cytoskeletal organization (PubMed:15601624). Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia (PubMed:14662653). Required for SRC-induced podosome formation (By similarity). Involved in positive regulation of immature dendritic cell migration (By similarity).
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Gene nameARHGEF5
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Uniprot IDQ12774
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Observed M.W.60 kDa
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