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Alternative name
EPM2B antibody
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Catalog
A005726
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman
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Applications Tested/SuitableELISA,WB,IHC
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PurificationImmunogen affinity purified
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ImmunogenNHL repeat containing 1
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IHCImmunohistochemistry of paraffin-embedded human heart using A005726(NHLRC1 antibody) at dilution of 1:50
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Western BlotL02 cells were subjected to SDS PAGE followed by western blot with A005726(NHLRC1 antibody) at dilution of 1:400
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Recommended dilutionWB : 1:200-1:2000
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Product Description specificalE3 ubiquitin-protein ligase. Together with the phosphatase EPM2A/laforin, appears to be involved in the clearance of toxic polyglucosan and protein aggregates via multiple pathways. In complex with EPM2A/laforin and HSP70, suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Ubiquitinates the glycogen-targeting protein phosphatase subunits PPP1R3C/PTG and PPP1R3D in a laforin-dependent manner and targets them for proteasome-dependent degradation, thus decreasing glycogen accumulation. Polyubiquitinates EPM2A/laforin and ubiquitinates AGL and targets them for proteasome-dependent degradation. Also promotes proteasome-independent protein degradation through the macroautophagy pathway.
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Gene nameNHLRC1
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Uniprot IDQ6VVB1
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Observed M.W.45-47kd
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