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Alternative name
AD4BP, FTZF1, SF1 antibody
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Catalog
A005847
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IP
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PurificationImmunogen affinity purified
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Immunogennuclear receptor subfamily 5, group A, member 1
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IHCImmunohistochemistry of paraffin-embedded human liver using A005847(NR5A1 antibody) at dilution of 1:100
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Western Blotmouse ovary tissue were subjected to SDS PAGE followed by western blot with A005847(NR5A1 antibody) at dilution of 1:1000
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Recommended dilutionWB:1:500-1:5000;IP:1:200-1:1000;IHC:1:20-1:200
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Product Description specificalTranscriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO- NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
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Gene nameNR5A1
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Uniprot IDQ13285
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Calculated M.W.52kDa
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Observed M.W.53 kDa
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