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Alternative name
CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1 antibody
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Catalog
A006067
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,IHC,IF,WB
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PurificationImmunogen affinity purified
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Immunogencyclin-dependent kinase inhibitor 1A (p21, Cip1)
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IHCImmunohistochemistry of paraffin-embedded human kidney using A006067(P21 antibody) at dilution of 1:50
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Western BlotHEK-293 cells were subjected to SDS PAGE followed by western blot with A006067(P21 antibody) at dilution of 1:500
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Recommended dilutionWB:1:500-1:5000;IP:1:200-1:1000;IHC:1:50-1:500;IF:1:20-1:200
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Product Description specificalCDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
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Gene nameCDKN1A
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Uniprot IDP38936
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Calculated M.W.21kDa
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Observed M.W.21 kDa
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