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Alternative name
NFKB 3, NFKB p65, NFKB3, p65, RELA, Transcription factor p65 antibody
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Catalog
A006089
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat,Pig
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Applications Tested/SuitableELISA,IHC,IF,IP,WB,FC
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PurificationImmunogen affinity purified
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Immunogenv-rel reticuloendotheliosis viral oncogene homolog A (avian)
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IHCImmunohistochemistry of paraffin-embedded human liver cancer using A006089(p65 antibody) at dilution of 1:50
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Western BlotHeLa cells were subjected to SDS PAGE followed by western blot with A006089(p65 antibody) at dilution of 1:1000
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Recommended dilutionWB:1:500-1:5000;IP:1:500-1:5000;IHC:1:50-1:200;IF:1:10-1:100
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Product Description specificalNuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.
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locationCytoplasm,Nucleus
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Uniprot IDQ04206
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Calculated M.W.65kDa
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